Journal: The Medical Bulletin of Sisli Etfal Hospital

Article Title: Neutrophil Elastase Inhibitor Increases Flap Survival in Experimental Degloving Injuries

doi: 10.14744/SEMB.2018.45077

Figure Lengend Snippet: (a) Immunohistochemical staining for the endothelial marker of angiogenesis CD31 were assessed in the control group. Arrow shows capillary formation stained with CD31. (b) Immunohistochemical staining for the endothelial marker of angiogenesis CD31 were assessed in the pentoxifylline group. Arrow shows capillary formation stained with CD31. (c) Immunohistochemical staining for the endothelial marker of angiogenesis CD31 were assessed in the sivelestat group. Sivelestat treated group showed an increase in the capillaries to approximately 200% of baseline from saline treated rats. Arrow shows capillary formation stained with CD31.

Article Snippet: The sections were incubated overnight at 4 oC with polyclonal goat anti-PECAM-1 (CD31) (#sc1506; SANTA CRUZ), (diluted 1:100 in 0.1M phosphate-buffered saline) that were detected with Alexa Fluor 488 donkey anti-goat (A11055; INVITROGEN) secondary antibody.

Techniques: Immunohistochemical staining, Staining, Marker, Control, Saline

Journal: PLoS ONE

Article Title: Fucoidan Reduces Secretion and Expression of Vascular Endothelial Growth Factor in the Retinal Pigment Epithelium and Reduces Angiogenesis In Vitro

doi: 10.1371/journal.pone.0089150

Figure Lengend Snippet: VEGF secretion was investigated in RPE/choroid organ culture (A) and ARPE-19 cell culture (B). RPE/choroid perfusion organ cultures were treated with 100 µg/ml fucoidan for 3 days and supernatant was collected at 6 hours, 24 hours and 3 days for one hour. ARPE-19 cells were treated with 100 µg/ml fucoidan for five days, and medium was collected after 1 day, 3 days and 5 days. VEGF content was evaluated with ELISA. Fucoidan reduced VEGF content compared to control in organ culture after 24 hours and 3 days (A). In cell culture, a reduction of VEGF secretion can be found after 3 days and 5 days (B). Significance was determined with student's t-test, + p<0.05; ++ p<0.01.

Article Snippet: The blot was treated with the first antibodies, against beta-actin (Cell Signaling Technologies) or VEGF (A-20) (Santa Cruz Biotechnology), overnight at 4°C in 2% skim milk in Tris buffered saline with 0.1% Tween.

Techniques: Organ Culture, Cell Culture, Enzyme-linked Immunosorbent Assay, Control

Journal: PLoS ONE

Article Title: Fucoidan Reduces Secretion and Expression of Vascular Endothelial Growth Factor in the Retinal Pigment Epithelium and Reduces Angiogenesis In Vitro

doi: 10.1371/journal.pone.0089150

Figure Lengend Snippet: Primary RPE cells (A) and ARPE-19 cells (B) were treated with 100 µg/ml fucoidan for 24 hours and the expression of intracellular VEGF (still containing signal peptide) was evaluated using immunocytochemistry. Cells treated with fucoidan exhibited a substantial decrease in intracellular VEGF expression.

Article Snippet: The blot was treated with the first antibodies, against beta-actin (Cell Signaling Technologies) or VEGF (A-20) (Santa Cruz Biotechnology), overnight at 4°C in 2% skim milk in Tris buffered saline with 0.1% Tween.

Techniques: Expressing, Immunocytochemistry

Journal: PLoS ONE

Article Title: Fucoidan Reduces Secretion and Expression of Vascular Endothelial Growth Factor in the Retinal Pigment Epithelium and Reduces Angiogenesis In Vitro

doi: 10.1371/journal.pone.0089150

Figure Lengend Snippet: ARPE-19 cells were treated with 250 µg/ml bevacizumab and 100 µg/ml fucoidan for 1 day, 5 days and 7 days. Controls cells were treated with bevacizumab only. Western blot for intracellular VEGF (still containing signal peptide) depicted a reduction of VEGF165 in Western blot at day 5 and day 7 (A), which was significant in densitrometric evaluation (B). Significance was determined with student's t-test, + p<0.05; +++ p<0.001. beva = bevacizumab, fuco = fucoidan.

Article Snippet: The blot was treated with the first antibodies, against beta-actin (Cell Signaling Technologies) or VEGF (A-20) (Santa Cruz Biotechnology), overnight at 4°C in 2% skim milk in Tris buffered saline with 0.1% Tween.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Fucoidan Reduces Secretion and Expression of Vascular Endothelial Growth Factor in the Retinal Pigment Epithelium and Reduces Angiogenesis In Vitro

doi: 10.1371/journal.pone.0089150

Figure Lengend Snippet: (A) Morphological appearance of OEC grown on Matrigel and stained with Calcein-AM which is converted to a green fluorescence by viable cells. Results indicated angiogenic structures in OEC treated with conditioned medium from RPE cells from different donors, VEGF and the EGM-2 (no VEGF). Additional treatment with fucoidan resulted in the reduction of vascular structures. (B) Quantitative image analysis depicting the skeleton length of angiogenic structures. Significance was determined with student's t-test, ++ p<0.01.

Article Snippet: The blot was treated with the first antibodies, against beta-actin (Cell Signaling Technologies) or VEGF (A-20) (Santa Cruz Biotechnology), overnight at 4°C in 2% skim milk in Tris buffered saline with 0.1% Tween.

Techniques: Staining, Fluorescence